Here is one of the major hero groups that shall be in Cartoon & Anime Heroes vs Villains.
- Stan Smith (American Dad)
- Avery Bullock (American Dad)
- Reginald Koala (American Dad)
- Dick Reynolds (American Dad)
- Jackson (American Dad)
- Duper (American Dad)
- John Sanders (American Dad)
- Akame (Akame ga Kill!)
- Tatsumi (Akame ga Kill!)
- Najenda (Akame ga Kill!)
- Lubbock (Akame ga Kill!)
- Leone (Akame ga Kill!)
- Bulat (Akame ga Kill!)
- Mine (Akame ga Kill!)
- Sheele (Akame ga Kill!)
- Susanoo (Akame ga Kill!)
- Chelsea (Akame ga Kill!)
- The Joes (GI Joe)
- Ben Tennyson (Ben 10)
- Kevin Levin (Ben 10)
- Gwen Tennyson (Ben 10)
- Rook Blonko (Ben 10)
- Rokuro "Rock" Okajima (Black Lagoon)
- Rebecca "Revy" Lee (Black Lagoon)
- Benny (Black Lagoon)
- Dutch (Black Lagoon)
- Sir Integra Fairbrook Wingates Hellsing (Hellsing)
- Walter C. Dornez (Hellsing)
- Pip Bernadotte (Hellsing)