Pinky and The Brain are two genentically-altered lab mice whom due to a gene-splicing incident, gained the ability of speech and thought. While Brain uses his incredible intellect to plot for world domination, Pinky enjoys the simple things of life like cheese, lint and fez hats. The two mice are the "antagonists" of Animaniacs and the spin-off series, Pinky and The Brain. The two are secondary players in the Non Disney Villains Tournament.
Non Disney Villains Tournament
Pest Control
Brain resolves to take over NIMH, only to be thwarted at every turn by Martin Brisby. Irritated by Martin's insanity, Brain decides to take matters into his own hands. He thus shoots and kills his opponent with a laser.
Friends and Enemies
Brain joins forces with Mojo Jojo, another animal experimented upon by Mok Swagger. The two, along with their other allies, scheme to topple Mok's dictatorial reign over the animals, with Brain serving as the chief inventor of the faction. After the murderous Scarface attempts to kill the Brain's ally, General Woundwort, the Brain deduces his foe's loyalties: Napoleon must have ordered the assassination. Brain teams up with Drake and another associate, Jenner, to take down some of Napoleon's allies: Cat R. Waul and Warren T. Rat. During the battle, Pinky fires some fireworks off at Warren T. Rat and his cats. Brain further frightens away Warren T. Rat and crew, leading Warren T. Rat to be murdered by Jenner. Cat R. Waul is also defeated, scoring the Brain and his allies a victory.
Serving Mechanicles
In "War of the Villains", Both Pinky and The Brain were used as the power source to Mechanicles' latest invention to clean the deserts of Agrahbah. Fed up by his mistreatment, Brain freed himself and Pinky from their cage to plot vengance on Mechanicles. While Pinky wondered how Brain would do it, Brain explained he would overpower Mechanicles' mechinizim. He was successful in his attempt and he and Pinky escaped as the vessel soared away into the sky.