The Owl Minions are the henchmen of the cruel and vain villain, the Grand Duke of Owls, and the secondary villains of the animated film, Rock-a-Doodle. They are minor players in the Non-Disney Villains Tournament, in Heroes vs. Villains War, and in Non-Disney Heroes vs. Villains War.
Non-Disney Villains Tournament
The Owl Minions briefly appear in the two occassions in the tournament. Initially, they assist their leader to his fight with Septimus, though they are not involved in the battle. On other note, the Owl Minions are featured in a meeting with Myotismon, the Grand Duke's actual master, who tasks the Grand Duke to retrieve some artifacts from the eccentric circus of Professor Screweyes. They do not make another appearance in this tournament.
Heroes Vs Villains War
A Lost Meal
When the League of Gentlemice enter the Grand Duke's domain, has his Owl Minions to eat them. Though, they manage to scare them off some of them, the Owl Minions get owned by Jaq, who knocks them out of his way. They accept defeat, after the Grand Duke is blasted out of his cavern.
Humans and Animals
They later make an appearance, when the League of Gentlemice enter Ratigan's new base, promting the villains to attack. In the end of the battle, the Grand Duke of Owls has his minions to capture several of the mice, after they finally accept defeat.